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poly da dt  (InvivoGen)


Bioz Verified Symbol InvivoGen is a verified supplier
Bioz Manufacturer Symbol InvivoGen manufactures this product  
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    Structured Review

    InvivoGen poly da dt
    Poly Da Dt, supplied by InvivoGen, used in various techniques. Bioz Stars score: 97/100, based on 448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/poly+da+dt/Poly(dA%3AdT)+LyoVec/us12649001-219-13-17
    Average 97 stars, based on 448 article reviews
    poly da dt - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Sterility:

    Article Title: Immune gene diversity and STING1 variants in shaping cancer immunity across different genetic ancestry populations
    Article Snippet: RBN2397 (Cat No: S8993) was acquired from Selleckchem and solubilized in DMSO. .. 2’3’-cGAMP (Cat No: tlrl-nacga23–1) and poly (dA:dT) (Cat No: tlrl-patn-1) were procured from Invivogen and dissolved in sterile, endotoxin-free water. ..

    Transfection:

    Article Title: Complexes for delivery of antigenic peptides
    Article Snippet: Briefly, 96-well plates were coated with poly-L-lysine solution (Cat. P8920, Sigma-Aldrich, St Louis, MO) before 1×104 HEK-293T cells were plated overnight and transfected with 25 ng of ISRE-luciferase reporter and titrating doses of pcDNA3.3-SOX2 using Lipofectamine 2000 (Cat. 11668019, Thermo Fisher Scientific, Waltham, NY). pcDNA3.1 was added to keep the amounts of DNA between well constant. .. The next day, cells were transfected with 50 ng of STING plasmid or poly (dA:dT) (Cat. tlrl-patn-1, InvivoGen, San Diego, CA) and harvested 16 hrs post-transfection. .. Cells were lysed in Luciferase cell culture lysis buffer (Cat. E1531, Promega, Madison, WI), and incubated with luciferase assay buffer (15 mM potassium phosphate (pH 7.8), 25 mM glycylglycine, 15 mM MgSO4, 4 mM EGTA, 2 mM ATP, 1 mM DTT) and luciferin solution (0.2 mM D-luciferin [Cat. L6882, Sigma-Aldrich, St Louis, MO], 15 mM MgSO4, 25 mM glycylglycine, 2 mM DTT).

    Plasmid Preparation:

    Article Title: Complexes for delivery of antigenic peptides
    Article Snippet: Briefly, 96-well plates were coated with poly-L-lysine solution (Cat. P8920, Sigma-Aldrich, St Louis, MO) before 1×104 HEK-293T cells were plated overnight and transfected with 25 ng of ISRE-luciferase reporter and titrating doses of pcDNA3.3-SOX2 using Lipofectamine 2000 (Cat. 11668019, Thermo Fisher Scientific, Waltham, NY). pcDNA3.1 was added to keep the amounts of DNA between well constant. .. The next day, cells were transfected with 50 ng of STING plasmid or poly (dA:dT) (Cat. tlrl-patn-1, InvivoGen, San Diego, CA) and harvested 16 hrs post-transfection. .. Cells were lysed in Luciferase cell culture lysis buffer (Cat. E1531, Promega, Madison, WI), and incubated with luciferase assay buffer (15 mM potassium phosphate (pH 7.8), 25 mM glycylglycine, 15 mM MgSO4, 4 mM EGTA, 2 mM ATP, 1 mM DTT) and luciferin solution (0.2 mM D-luciferin [Cat. L6882, Sigma-Aldrich, St Louis, MO], 15 mM MgSO4, 25 mM glycylglycine, 2 mM DTT).

    other:

    Article Title: Histone demethylase KDM4B epigenetically controls NLRP3 expression to enhance inflammatory responses
    Article Snippet: Poly (dA:dT) (200 ng/mL) , Invivogen , tlrl-patn.

    Article Title: Conjugated bile acids facilitate cholangiocyte senescence to promote cholestatic liver diseases via STING signaling.
    Article Snippet: Poly (dA:dT) (Tlrl-patn) and Lipofectamine 3000 (L3000008) were gained from Invivogen Ltd., USA.

    Western Blot:

    Article Title: IFI16/204 Promotes Dendritic Cell Activation and Anti-Hepatocellular Carcinoma Efficacy via the STING-TBK1-IRF3 Signalling Pathway.
    Article Snippet: IFI16 (the murine homologue is IFI204) is an intracellular doublestranded DNA (dsDNA) pattern recognition receptor (PRR) that plays a crucial role in bridging innate and adaptive immunity.. However, its function in dendritic cell (DC) activation and antihepatocellular carcinoma (HCC) efficacy remains poorly characterised.. This study demonstrates that IFI16 promotes DC maturation, functional activation and antitumor immunity.



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